Measurement of Serum Lactic Dehydrogenase
نویسنده
چکیده
A colorimetric method is described for the measurement of serum lactic dehydrogenase (LDH) activity. The method employs phenazine methosulfate to mediate electron transfer between reduced diphosphopyridinenucleotide and 2,6-dichloroindophenol,and allows continuous measurement of the reaction rate in the direction lactate to pyruvate. The LDH of a number of normal and pathologic sera was measuredby the present method and by the ultraviolet spectrophotometricmethod. Agreement between the two methods is good.
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تاریخ انتشار 2004